Use of a single procedure for selective enrichment, isolation, and identification of plasmid-bearing virulent Yersinia enterolitica of various serotypes from pork samples

Article Abstract:

A novel recovery method was developed for the enrichment and isolation of Yersinia enterocolitica from infected pork samples. The recovery method involved the presumptive isolation of Yersinia enterocolitica strains on MAC and CIN agars. The virulent plasmid-bearing clones were identified via Congo red binding technique and low-calcium response techniques. Furthermore, multiplex polymerase chain reaction was utilized to verify the virulent strains of Yersinia enterocolitica.

Methods, Analysis, Identification and classification, Bacteriology

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Direct detection and isolation of plasmid-bearing virulent serotypes of yersinia enterocolitica from various foods

Article Abstract:

Research was conducted to study a method developed for the detection and isolation of various trains of Yersinia enterocolitica (YEP+) serotypes from different food sources. YEP+ cultures were grown in BHHI broth for 18 hours at 25 degrees C while enrichment bags were put in a shaking incubator at 12 degrees C. Results showed that the method eliminated a day of enrichment and another for the presumptive isolation while preserving the virulence plasmid.

Plasmids

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Survival of cold-stresses campylobacter jejuni on ground chicken and chicken skin during frozen storage

Article Abstract:

The effects of refrigerator at 4 degree Celsius and a combination of refrigerator and freezing on the survival of campylobacter jejuni in ground chicken and on chicken skin are investigated. The findings indicate that, individually or in combination, refrigeration and freezing are not a substitute for safe handling and proper cooking of poultry.

Science & research, Campylobacter, Chicken (Meat)

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