Use of molecular and isotopic techniques to monitor the response of autotrophic ammonia-oxidizing populations of the beta subdivision of the class Proteobacteria in arable soils to nitrogen fertilizer

Article Abstract:

Research was conducted to investigate the impact of NH4NO3 fertilizer on the size and activity of nitrifying, autotrophic, ammonia-oxidizing populations of the beta subdivision of the class Proteobacteria in arable soils. Samples were obtained from arable plots under different long-term fertilizer regimes before and after NH4NO3 addition. 15N isotopic pool dilution assays were used to estimate the rates of nitrification. Results indicate a population dominated by Nitrosospira-type organisms comparable to those prevalent in other soils.

Analysis, Soil research, Nitrification, Nitrogen fertilizers

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Oxidation of reduced inorganic sulfur compounds by bacteria: emergence of a common mechanism?

Article Abstract:

There may be a common mechanism for sulfur oxidation in lithotrophic and phototrophic sulfur-oxidizing bacteria, according to researchers who studies Paracoccus pantotrophus. In this bacterium, sulfur binds to the SoxY protein, the sulfur is oxidized by sulfur dehydrogenase Sox CS, and sulfate is hydrolyzed by sulfatase SoxB.

Sulfur bacteria

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Autotrophic ammonia oxidation at low pH through urea hydrolysis

Article Abstract:

Urea hydrolysis appears to be the mechanism that allows some bacteria to nitrify at a low pH. These autotrophic ammonia oxidizers use urea as a source of ammonia, as documented by a study of Nitrosospira sp. strain NPAV.

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