Direct proteomic mapping of the lung microvascular endothelial cell surface in vivo and in cell culture

Article Abstract:

Endothelial cells can function differently in vitro and in vivo, however, the degree of micro environmental modulation in vivo remains unknown at the molecular level mainly due to the analytical limitations. Multidimensional protein identification technology (MudPIT) were used to identify 450 proteins (with three or more spectra) in luminal endothelial cell plasma membranes in vivo are not detected in vitro.

Methods, Cell culture, Properties, Glycocalyx

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Live dynamic imaging of caveolae pumping targeted antibody rapidly and specifically across endothelium in the lung

Article Abstract:

Quantitative proteomics are used to identify aminopeptidase P (APP) concentrated in caveolae of lung endothelium, which shows that APP antibody targets nanoparticles to caveolae. In vivo proteomic imaging has integrated organellar proteomics with multiple imaging methods in order to identify an accessible target space that includes the transvascular pumping space of the caveola.

Usage, Medical examination, Endothelium, Diagnostic imaging

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Vascular targeting via caveolae

Article Abstract:

The novel imaging techniques show that caveolae build up transcytotic pumps that facilitate the transport of antibodies from the blood into the lung in rats. This finding can be useful in the development of cancer treatment to arrest metastases by destroying blood supply to tumor.

Physiological aspects, Lungs, Lung, Biological transport, Viral antibodies, Antibodies

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