Femtosecond spectroscopy of halorhodopsin and rhodopsin in a broad spectral range of 400-1000 nanometers

Article Abstract:

The transient transmittance spectra and femtosecond to picosecond transition kinetics in samples of rhodopsin from octopus and halorhodopsin from the Halobacterium halobium were examined. Spectroscopic analyses using a colliding-pulse mode-locked dye laser evaluated the excited states, the gain and absorption spectra of both samples. The findings revealed a coexistence between the long-wavelength gain with longer lifetime than the formation of the primerhodopsin and the J intermediate. A model of ballistic and diffusive channels was proposed to explain the isomerization in rhodopsins.

Analysis, Absorption, Isomerization, Molecular spectroscopy

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Base complementarity and nucleoside recognition in phosphatidylnucleoside vesicles

Article Abstract:

A study was conducted to evaluate molecular interactions exhibited by liposomes in phosphatidylnucleosides with complementary structures. Spectroscopic analyses were used to identify liposome shape, size and distribution. Fusion was selected as the mechanism of liposome interaction. The findings demonstrate base-base interactions in the nucleoside samples. Moreover, a 1:1 ratio was observed in the liposomes of complementary phosphatidylnucleosides. The data also confirmed that vesicle organization facilitates base-base interactions.

Liposomes, Nucleosides, Complementarity (Physics)

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Modeling the 3D structure of rhodopsin using a de novo approach to build G-protein-coupled receptors

Article Abstract:

A study was conducted to analyze a novel technique of constructing three-dimensional structures of the transmembrane domain of G-protein-coupled receptors. The technique integrates knowledge of the amino acid sequence and utilizes the helix spatial arrangement of rhodopsin determined from its low-resolution electron density map. A three-dimensional framework of bacteriorhodopsin was made using a low-resolution electron density map of the protein.

G proteins, Amino acids, Bacteriorhodopsin

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