Tyrosyl rotamer interconversion rates and the fluorescence decays of N-acetyltyrosinamide and short tyrosyl peptides

Article Abstract:

The rotameric populations and interchange rates for tyrosine in N-acetyltyrosinamide (NATyrA), the tripeptide Tyr-Gly-Gly (YGG), and the pentapeptide Leu-enkephalin (YGGFL) are measured. The analysis has shown that temperature dependence of the rotamer interchange rate increased with increasing peptide length, suggesting an increased barrier due to steric hindrance.

Science & research, Research, Peptides, Peptide synthesis, Amides, Tyrosine metabolism

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Single molecule tracking of sub-millisecond domain motion in calmodulin

Article Abstract:

A singlepair fluorescence resonance energy transfer (spFRET) is used to track distance changes between domains of fluorescently labeled calmodulin (CaM) on the sub-millisecond time scale. It is concluded that the interchange of the conformational substrates of CaM is rare on the microsecond time scale.

Calmodulin, Spectra, Fluorescence spectroscopy, Energy transformation

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Single-molecule resonance energy transfer and fluorescence correlation spectroscopy of calmodulin in solution

Article Abstract:

Single molecule Forster resonance energy transfer (FRET) between the two domains are studied and reported. The FRET efficiency distribution by analysis of fluorescence bursts in solutions are measured.

Fluorescence, Energy transfer

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