An automated approach to generating expressed sequence catalogues

Article Abstract:

A new automated system for the large-scale examination of complementary DNA (cDNA) libraries will allow all expressed sequences of an organism to be cataloged. The system also has the long-range goal of allowing sequencing by hybridization, which is expected to be less limited in application than the gel-based sequencing currently in use. The new automated system relies on 384 wells for plate storage and handling, a colony picker, high-throughput polymerase chain reactions and a spotting robot for analysis of clones.

Analysis, Technology application, Nucleotide sequence, Base sequence, Genetic research

---

A novel thermostable polymerase for DNA sequencing

Article Abstract:

Thermo Sequenase is an innovative thermostable DNA polymerase that improves upon the T7 Sequenase DNA polymerase while maintaining a comparable base calling accuracy. Cycle sequencing's reliability and sensitivity is incorporated into the base calling accuracy through specific engineering for DNA sequencing. Some improvements involve preventing 5'-3' exonuclease activity through the addition of thermostable inorganic pyrophosphatase.

Research, Escherichia coli, DNA polymerases

---

Similar abstracts:

• Abstracts: Time, money and tradeoffs. An alternative approach to establishing trade-offs among greenhouse gases. Light at the end of the tunnel
• Abstracts: An arid-adapted middle Pleistocene vertebrate fauna from south-central Australia. Ice shelves in the Pleistocene Arctic Ocean inferred from glaciogenic deep-see bedforms
• Abstracts: Delaminated zeolite precursors as selective acidic catalysts. Direct measurement of potassium-promoted change in heat of adsorption of CO on Ni(100)
• Abstracts: Satellite ozone monitoring error. The crucial measurement
• Abstracts: Cloning and expression of apical membrane water channel of rat kidney collecting tubule. The evolution of apical dominance in maize

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.