DNA repair by recycling reverse transcripts

Article Abstract:

Double-stranded chromosomal breaks in Saccharomyces cerevisiae are often repaired by the insertion of yeast retrotransposon (Ty1) plus-strand DNA generated by reverse transcription from tRNA sequences. The Ty1 insertions associated with the primer-binding site may be derived from the plus-strand cDNAs whose 3' ends are templated by primer tRNA. This plus-strand strong-stop DNA, the most abundant product of the Ty1 reverse transcription process, remains unused in further Ty1 synthesis and is recycled by the yeast in processes such as the repair of chromosomal breaks.

DNA repair

---

A role for reverse transcripts in gene conversion

Article Abstract:

RNA-controlled gene conversion was carried out for the first time in genetic engineering research involving the yeast Saccharomyces cerevisiae. Conversion of the yeast's his3 reporter gene to its homologous chromosomal allele by insertion of complementary DNA proved that recombination between a reverse transcriptase and its homologue is possible. RNA-mediated gene conversion may help initiate genomic evolution through the loss of introns and change in gene families inherent in the process.

Innovations, Genetic regulation

---

The barley Hooded mutation caused by a duplication in a homeobox gene intron

Article Abstract:

The supposition that the Knox gene family is involved in floral evocation is supported by the influence of Knox3 overexpression in barley and heterologous tobacco. A 305-base pair duplication in intron 4 causes ectopic Knox3 gene expression in the primordium of an extra floret produced in the Hooded mutation. Knox3 represents the genetic locus K which is the only regulator of the Hooded mutation phenotype.

Mutation (Biology), Mutation

Similar abstracts:

• Abstracts: DNA fingerprint variation and reproductive fitness in the Plain pigeon. Remarkable amphibian biomass and abundance in an isolated wetland: implications for wetland conservation
• Abstracts: Arithmetic in the cradle. Addition and subtraction by human infants. Symbolic arithmetic knowledge without instruction
• Abstracts: Vesicle assembly in microtubes. Toxin-induced activation of the G protein p21 Rho by deamidation of glutamine
• Abstracts: A role for retinoblastoma protein in potentiating transcriptional activation by the glucocorticoid receptor. Myc and Max proteins possess distinct transcriptional activities
• Abstracts: Threatened native American plants. The elimination of the Category 2 candidate species list: a prescription for environmental train wrecks

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.