Structure of the trp RNA-binding attenuation protein, TRAP, bound to RNA

Article Abstract:

Research revealing the crystal structure of a complex of the trp RNA-binding attenuation protein and a 53-base single-stranded RNA containing 11 GAG triplets indicates that the recognition of single-stranded RNA does not necessarily involve protein interactions with the phosphates of RNA. Furthermore, the structure indicates that some RNA segments are bound to protein with the same conformational angles that arise in the A-form double helix. This suggests that TRAP may choose from the nascent RNA transcript the conformation likely to predominate before binding.

Protein binding, RNA

---

+A protein catalytic framework with an N-terminal nucleophile is capable of self-activation

Article Abstract:

Three enzymes and their capability for autocatalytic activation and engaging in nucleophilic attack are discussed. These three are glutamine PRPP amidotransferase, penicillin acylase and poteasome. They occupy a common fold that enables them to perform similar activities although they look divergent enough to mask their common evolutionary pattern and origin. A suggested name for this set of enzymes is Ntn hydrolases.

Enzymes

---

Penicillin acylase has a single-amino-acid catalytic centre

Article Abstract:

The crystal structure of penicillin acylase is determined at a resolution of 1.9 angstroms by using multiple isomorphous replacement technique. The crystal structure reveals that there are no adjacent histidine type molecules near the catalytically active N-terminal serine of the B-chain. The serine region has an alpha-amino group which provides the penicillin acylase enzyme with its catalytic properties.

Structure-activity relationships (Pharmacology), Penicillin, Penicillins

Similar abstracts:

• Abstracts: Structure of the single-stranded-DNA-binding domain of replication protein A bound to DNA. Crystal structure of the CorA M[g.sup.2+] transporter
• Abstracts: Modification by nuclear export? Structure of the DNA-binding domains from NFAT, Fos and Jun bound specifically to DNA
• Abstracts: A structural change in the kinesin motor protein that drives motility. How kinesin waits between steps
• Abstracts: Do female pigeons trade pair copulations for protection? Sex roles and sexual selection. Depletion determines sperm number in male zebra finches
• Abstracts: Size and stability of vertebrate leks. Measuring sexual selection. Lek size, male mating skew and the evolution of lekking

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.