Dependence of Trichomonas vaginalis upon polyamine backconversion

Article Abstract:

Trichomonas vaginalis, a urogenital parasite, and its dependence on polyamine backconversion are discussed as is the potential of bis(alkyl) polyamine analogs as antitrichomonad agents. A study has shown that growth of T. vaginalis with DENSpm also brings on polyamine depletion and significant inhibition of growth by a mechanism that involves competition with the transport of spermine and inhibition of SSAT effectively blocking spermidine synthesis. The parasite differs significantly from other eukaryotes in several aspects of its polyamine metabolism. The spermine taken up is backconverted to spermidine through a spermidine:spermine N(super.1)-acetyltransferase (SSAT)/polyamine oxidase-coupled pathway.

Diseases, Parasites, Trichomonas vaginalis, Trichomonas, Protozoa, Protozoans, Genitourinary organs, Urogenital system

---

Eubacterial arylamine N-acetyltransferases: identification and comparison of 18 members of the protein family with conserved active site cysteine, histidine and aspartate residues

Article Abstract:

Eubacterial arylamine N-acetyltransferases (NATs) are discussed based on identification and comparison of 18 members of the protein family. A study was undertaken with the goal of determining how widespread NAT is in the prokaryotes. Also of interest was to what extent conserved residues are maintained. Conserved active site cysteine, histidine, and aspartate residues were found.

United Kingdom, Reports, Catalysts, Eukaryotic cells, Prokaryotes, Cells (Biology), Eukaryotes, Binding sites (Biochemistry), Active sites (Biochemistry), Microbiological research

---

Reconstitution of a bacterial/plant polyamine biosynthesis pathway in Saccharomyces cerevisiae

Article Abstract:

A study was conducted to analyze the reconstitution of a bacterial/plant polyamine biosynthesis route in Saccharomyces cerevisiae. The ABI 377 automated sequencer was utilized to carry out nucleic acid sequence analysis. Several yeast vectors were then prepared to evaluate the complementation of polyamine biosynthesis. In addition, yeast strains supporting knock-out mutations and multiple auxotrophic mutations were prepared using the random spore analysis method.

Bacteria, Biosynthesis, Saccharomyces, Yeast fungi, Yeasts (Fungi)

Similar abstracts:

• Abstracts: stcS, a putative P-450 monooxygenase, is required for the conversion of versicolorin A to sterigmatocystin in Aspergillus nidulans
• Abstracts: CodY-regulated aminotransferases AraT and BcaT play a major role in the growth of Lactococcus lactis in milk by regulating the intracellular pool of amino acids
• Abstracts: Molecular cloning, sequencing, and expression of omp-40, the gene coding for major outer membrane protein from the acidophilic bacterium Thiobacillus ferrooxidance
• Abstracts: Diversified transcription initiation complexes expand promoter selectivity and tissue-specific gene expression
• Abstracts: Identification and purification of two distinct complexes containing the five RAD51 paralogs. The p120 catenin partner Kaiso is a DNA methylation-dependent transcriptional repressor

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.