Purification and characterization of dimethylsulfoniopropionate lyase from an Alcaligenes-like dimethyl sulfide-producing marine isolate

Article Abstract:

Vitek gram-negative identification method helps identify dimethylsulfoniopropionate (DMSP) lyase from an anaerobic gram-negative rod-shaped marine bacterium, Alcaligenes. Hydrophobic chromatography helps purify the DMSP lyase to electrophoretic homogeneity. Analysis of DMSP lyase reveals it as a 48-kDa monomer and it exhibits a Michaelis-Menten constant of 1.4 mM and a V(max) of 408 micromol min mg of protein. DMSP lyase stoichiometrically converts DMSP to dimethyl sulfide and acrylate.

Analysis, Microbial enzymes

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Comparative physiology of dimethyl sulfide production by dimethylsulfoniopropionate lyase in Pseudomonas doudoroffii and Alcaligenes sp. strain M3A

Article Abstract:

The velocity of enzymatic degradation of dimethylsulfoniopropionate (DMSP) by DMSP lyase yielding dimethyl sulfide in the salt marsh bacterium Alcaligenes species is three times higher than that of the marine bacterium Pseudomonas (P.) doudoroffii. The enzyme is unstable under aeration, its activity increases with salinity of medium, and has a single peak at pH 8 in P. doudoroffii.

Physiological aspects, Enzymes, Enzyme regulation, Dimethyl sulfide

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Nuclear magnetic resonance analysis of [1-(super 13)C]dimethylsulfoniopropionate (DMSP) and [1-(super 13)C]acrylate metabolism by a DMSP lyase-producing marine isolate of the alpha-subclass of Proteobacteria

Article Abstract:

In the alpha-Proteobacteria, dimethylsulfoniopropionate (DMSP) is metabolized by intracellular enzymes whereas acrylate is metabolized on the cell surface. The intracellular lyase is induced by DMSP, acrylate, and beta-hydroxypropionate.

Microbial metabolism

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