Reduced X-ray resistance and homologous recombination frequencies in a RAD54-l- mutant of the chicken DT40 cell line

Article Abstract:

The rad54 mutants of Saccharomyces cerevisiae are very sensitive to X-ray and exhibit decreased mitotic recombination frequencies due to a defect in double-strand break repair. Immunoglobulin gene conversion of the chicken B cell line DT40 resulted in the disruption of an RAD54 homolog. The RAD54-l- disruption mutants in DT40 exhibited a phenotype similar to that of rad54 yeast mutants. This phenotype provides the first genetic evidence of a relationship between double-strand break repair and homologous recombination in vertebrate cells.

Phenotype, Phenotypes, Saccharomyces, Chickens, Cell lines

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Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells

Article Abstract:

A novel strategy of the first reconstruction of an open reading frame for a salmonoid transposase and systematic introduction of amino acid replacements were illustrated. The transposon system was named Sleeping Beauty (SB) can be developed as an instrument for germline transformation and insertional mutagenesis in vertebrates. The effectiveness of phylogenetic approach for constructing the active transposon was demonstrated by the activities of SB.

Mutagenesis

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Ancient missense mutations in a new member of the RoRet gene family are likely to cause familial Mediterranean fever

Article Abstract:

A gene from a 115-kb candidate interval on the 16p chromosome was cloned to determine the genetic causes of the familial Mediterranean fever. Affected individuals were found to exhibit three varying missense mutations. Mutational and haplotype studies also reveal that populations that have been separated for centuries have carrier chromosomes with ancestral relationships.

Clone cells, Familial Mediterranean fever, Periodic disease

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