Site-specific RNase E cleavage of oligonucleotides and inhibition by stem-loops

Article Abstract:

Experimental studies on 10-13 nucleotides which have the RNase E-cleaved sequence of RNA I but lack the stem-loop indicate that the nucleotides are cleaved at the same phosphodiester bond as RNA I and the cleavage is 20 times more efficient than that of RNA I. RNase E does not enter via stem-loops, which merely restrict cleavage at susceptible sites. Mutations in a non-adjacent stem-loop restrict cleavage, indicating that enzyme access is affected by distant conformational changes.

Enzymes, RNA splicing, Nucleotides, Enzyme regulation

---

RNA degradation in Escherichia coli regulated by 3' adenylation and 5' phosphorylation

Article Abstract:

The digestion rate of RNA1 by the polynucleotide phosphorylase, 3'exonuclease is dependent on the 5' phosphorylation and 3'adenylation in Escherichia coli. The Escherichia coli the polyadenylation process of digestion is mediated by the pcn B gene. This gene enhances the speed of RNA I decay, thereby increasing the rate of digestion. The process of polyadenylation also results in the formation of mRNA type characteristics to the decaying RNA I.

Phosphorylation

---

Poly(A)-and poly(U)-specific RNA 3 tail shortening by E. coli ribonuclease E

Article Abstract:

The ribonuclease (RNase) E enzyme from Escherichia coli is extensively studied. Its endoribonuclease activity on single-stranded RNA has a significant role in ribosomal RNA processing. It is now reported that RNase E has the capability to short 3' poly(A)- and poly(U)-homopolymer tails on RNA molecules. This suggests that RNase E may have overall control over RNA decay.

Observations, Ribonuclease

Similar abstracts:

• Abstracts: Autocatalytic RNA cleavage in the human beta-globin pre-mRNA promotes transcription termination. Defects in RGS9 or its anchor protein R9AP in patients with slow photoreceptor deactivation
• Abstracts: Direct activation of inward rectifier potassium channels by PIP2 and its stabilization by G BetaY. Clathrin's Achilles ankle
• Abstracts: Cyclin-dependent kinases regulate the antiproliferative function of Smads. The protein kinase A anchoring protein mAKAP coordinates two integrated cAMP effector pathways
• Abstracts: Site-specific chromosomal mutagenesis using the TargeTron(super TM) Gene Knockout System. Acute mutation of reinoblastoma gene function is sufficient for cell cycle re-entry
• Abstracts: Structural basis for the function and inhibition of an influenza virus proton channel. The mechanism by which influenza A virus nucleoprotein forms oligomers and binds RNA

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.